Grafted hPSC-derived GABA-ergic interneurons regulate seizures and specific cognitive function in temporal lobe epilepsy

Grafted hPSC-derived GABA-ergic interneurons regulate seizures and specific cognitive function in temporal lobe epilepsy

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Abstract Interneuron loss/dysfunction contributes to spontaneous recurrent seizures (SRS) in chronic temporal lobe epilepsy (TLE), and interneuron grafting A rapid dynamic headspace method for authentication of whiskies using artificial neural networks into the epileptic hippocampus reduces SRS and improves cognitive function.This study investigated whether graft-derived gamma-aminobutyric acid positive (GABA-ergic) interneurons directly regulate SRS and cognitive function in a rat model of chronic TLE.Human pluripotent stem cell-derived medial ganglionic eminence-like GABA-ergic progenitors, engineered to express hM4D(Gi), a designer receptor exclusively activated by designer drugs (DREADDs) through CRISPR/Cas9 technology, were grafted into hippocampi of chronically epileptic rats to facilitate the subsequent silencing of graft-derived interneurons.Such grafting substantially reduced SRS and improved hippocampus-dependent cognitive function.Remarkably, silencing of graft-derived interneurons with Geological and hydrometeorological hazards affecting livestock production in Ethiopia: a systematic review of impacts, mitigation, and adaptation strategies a designer drug increased SRS and induced location memory impairment but did not affect pattern separation function.

Deactivation of DREADDs restored both SRS control and object location memory function.Thus, transplanted GABA-ergic interneurons could directly regulate SRS and specific cognitive functions in TLE.